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发表于 2013-4-11 10:32:57
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Tranexamic acid detection
Tranexamic acid determination - and titration
Applications
This method uses titration tranexamic acid tablets tranexamic acid content.
This method is applicable tranexamic acid tablets.
Principle of the method
This method uses titration tranexamic acid tablets tranexamic acid content.
This method is applicable tranexamic acid tablets.
Equipment:
Sample Preparation: perchloric acid solution (0.1 mol / l)
Preparation: anhydrous glacial acetic acid (water content per gram of water plus acetic anhydride 5.22mL) 750ml, adding perchloric acid (70% -72%) 8.5 ml, shake, slowly added dropwise acetic anhydride at room temperature 23ml, plus side while shaking the addition was completed and then shake evenly, let cool, anhydrous acetic acid to make into a 1000ml, shake for 24 hours. Measured by the test easy to acetylation required moisture determination method for determination of the water content, water and acetic anhydride adjusted to the liquid water content of 0.01% -0.2%.
Calibration: 105 ℃ drying to constant weight basis of about 0.16 g of potassium hydrogen phthalate, accurately weighed, add anhydrous glacial acetic acid to dissolve 20ml, add crystal violet indicator solution, the liquid slowly titrated to blue, and the titration of the blank test correction. Per 1ml of perchloric acid solution (0.1 mol / l) is equivalent to 20.42mg of potassium hydrogen phthalate. According to the consumption of the liquid potassium hydrogen phthalate Take the amount, calculate the concentration of the solution, you can.
Storage: set brown glass bottles, sealed.
2 crystal violet indicator solution
Take the crystal violet 0.5g, add 100 ml of glacial acetic acid to dissolve, that was.
Reagents
1 water (boiling to room temperature)
Perchloric acid solution (0.1 mol / l)
(3) glacial acetic acid
4 benchmark potassium hydrogen phthalate
5 crystal violet indicator solution
Sample Preparation
Perchloric acid solution (0.1 mol / l)
Preparation: anhydrous glacial acetic acid (water content per gram of water plus acetic anhydride 5.22mL) 750ml, adding perchloric acid (70% -72%) 8.5 ml, shake, slowly added dropwise acetic anhydride at room temperature 23ml, plus side while shaking the addition was completed and then shake evenly, let cool, anhydrous acetic acid to make into a 1000ml, shake for 24 hours. Measured by the test easy to acetylation required moisture determination method for determination of the water content, water and acetic anhydride adjusted to the liquid water content of 0.01% -0.2%.
Calibration: 105 ℃ drying to constant weight basis of about 0.16 g of potassium hydrogen phthalate, accurately weighed, add anhydrous glacial acetic acid to dissolve 20ml, add crystal violet indicator solution, the liquid slowly titrated to blue, and the titration of the blank test correction. Per 1ml of perchloric acid solution (0.1 mol / l) is equivalent to 20.42mg of potassium hydrogen phthalate. According to the consumption of the liquid potassium hydrogen phthalate Take the amount, calculate the concentration of the solution, you can.
Storage: set brown glass bottles, sealed.
2 crystal violet indicator solution
Take the crystal violet 0.5g, add 100 ml of glacial acetic acid to dissolve, that was.
Procedure
20 to take this product, accurately weighed, small study, accurately weighed goods from the amount (equivalent to approximately the tranexamic acid 0.25g), add 40 ml of glacial acetic acid is dissolved, add 1 to 2 drops of crystal violet indicator solution with highperchloric acid titrant (0.1 mol / l) titration solution blue-green, and the titration of the blank test correction, record consumption of perchloric acid titrant volume (ml) per 1 ml of perchloric acid titration solution (0.1 mol / l) is equivalent to 15.72mg of tranexamic acid (C8H15NO2), that is, too.
Note 1: "Precision Weigh" means to check the weight should be accurately referred to take the weight of one-thousandth, "precise amount" means to measure volume accuracy shall conform to national standards for the volume pipette accuracy requirements. |
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